Review



prk7 ha s6k1  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc prk7 ha s6k1
    Prk7 Ha S6k1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk7+ha+s6k1+wt/pRK7-HA-S6K1-WT+(Plasmid+%238984)/pm41802695-76-27-28
    Average 93 stars, based on 44 article reviews
    prk7 ha s6k1 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: HMGA2 and protein leucine methylation drive pancreatic cancer lineage plasticity
    Article Snippet: .. After annealing following standard protocols, hybridized oligos were digested with MluI and EcoRI and subcloned into pRetroHMGA2. pRK7-HA-S6K1-WT and pRK7-HA-S6K1 E387∆CT were a gift from John Blenis (Addgene plasmid #8984 and #8993). .. HA-S6K1-WT and HA-S6K1 E387∆CT were subcloned into pRetroX-TIGHT-Blast plasmid using XbaI and EcoRI. pLVX-Tet-On was obtained from Clontech.

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome.
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. Cell culture and retroviral transduction MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO2.

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7-HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO 2 .

    Polymerase Chain Reaction:

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome.
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. Cell culture and retroviral transduction MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO2.

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7-HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO 2 .

    Amplification:

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome.
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. Cell culture and retroviral transduction MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO2.

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7-HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO 2 .

    Clone Assay:

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome.
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. Cell culture and retroviral transduction MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO2.

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7-HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO 2 .

    Retroviral:

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome.
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. Cell culture and retroviral transduction MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO2.

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7-HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO 2 .

    Sequencing:

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome.
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. Cell culture and retroviral transduction MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO2.

    Article Title: Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome
    Article Snippet: EcoHelper (pCL-Eco, Addgene) was used for the retroviral infection of MEFs. .. For the S6K rescue experiments, HA-tagged coding sequences of either WT or K100R S6K1 rat cDNA were PCR amplified from either pRK7-HA-S6K1-WT (Addgene, 8984) or pRK7-HA-S6K1-KR (Addgene, 8985) and cloned into EcoRI and SalI site of the retroviral vector pBABE-puro (Addgene, 1764) and sequence verified. .. MEFs were maintained in DMEM supplemented with EmbryoMax FBS (Millipore) and 1× antibiotic-antimycotic at 37 °C and 5% CO 2 .

    Generated:

    Article Title: SRC inhibition enables formation of a growth suppressive MAGI1-PP2A complex in isocitrate dehydrogenase-mutant cholangiocarcinoma
    Article Snippet: .. Human MAGI1c-Y373F–green fluorescent protein (GFP) mutants were generated from pCMV6-AC-GFP-MAGI1c WT (Origene, #RG212712) by site directed mutagenesis using the following primers: CATAGTAGATACCAAAGCAGGG TCTTCAATCTTTTCCCAAC and GTTGGGAAAAGATTGAA GACCCTGTCTTTGGTATCTACTATG. pCMV SRC WT and pRK7-HA-S6K1-WT (Addgene, #8984) were gifts from J. Cooper and J. Blenis. ..

    Mutagenesis:

    Article Title: SRC inhibition enables formation of a growth suppressive MAGI1-PP2A complex in isocitrate dehydrogenase-mutant cholangiocarcinoma
    Article Snippet: .. Human MAGI1c-Y373F–green fluorescent protein (GFP) mutants were generated from pCMV6-AC-GFP-MAGI1c WT (Origene, #RG212712) by site directed mutagenesis using the following primers: CATAGTAGATACCAAAGCAGGG TCTTCAATCTTTTCCCAAC and GTTGGGAAAAGATTGAA GACCCTGTCTTTGGTATCTACTATG. pCMV SRC WT and pRK7-HA-S6K1-WT (Addgene, #8984) were gifts from J. Cooper and J. Blenis. ..

    other:




    Similar Products

    93
    Addgene inc prk7 ha s6k1
    Prk7 Ha S6k1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk7+ha+s6k1+wt/pRK7-HA-S6K1-WT+(Plasmid+%238984)/pm41802695-76-27-28
    Average 93 stars, based on 1 article reviews
    prk7 ha s6k1 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc prk7 ha s6k1 wt
    Prk7 Ha S6k1 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk7+ha+s6k1+wt/pRK7-HA-S6K1-WT+(Plasmid+%238984)/pmc12106622__41467_2025_60129_MOESM1_ESM-46-17-27
    Average 93 stars, based on 1 article reviews
    prk7 ha s6k1 wt - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc control injectionmarkers pcfj90
    Control Injectionmarkers Pcfj90, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk7+ha+s6k1+wt/pRK7-HA-S6K1-WT+(Plasmid+%238984)/pm39946234-799-29-33
    Average 93 stars, based on 1 article reviews
    control injectionmarkers pcfj90 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc control injection markers pcfj90
    Control Injection Markers Pcfj90, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk7+ha+s6k1+wt/pRK7-HA-S6K1-WT+(Plasmid+%238984)/bio_rxiv__2024__08__31__610619-179-20-27
    Average 93 stars, based on 1 article reviews
    control injection markers pcfj90 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc prk7 ha s6k1 wt plasmid
    Prk7 Ha S6k1 Wt Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk7+ha+s6k1+wt/pRK7-HA-S6K1-WT+(Plasmid+%238984)/pmc11218711-807-9-14
    Average 93 stars, based on 1 article reviews
    prk7 ha s6k1 wt plasmid - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results